TR-FRET Kits

TR-FRET is the combination of TRF (Time-resolved fluorometry) that offer low background with FRET (Förster resonance energy transfer) that offers a homogeneous assay. It is a transfer of energy resulting from an interaction between two light- sensitive molecules (an acceptor and a donor). The transfer is possible thanks to the compatibility of the wavelengths of the two chromophores

Using our Bright-DtechTM as a donor, background noise will be reduced/suppressed. Composed of lanthanides, our nanoparticles have the particularity of having a long lifetime, i.e. they emit long fluorescence (several milliseconds). Thus, it is possible to have a delay between the excitation/emission of the nanoparticle and the reading. Thanks to this delay, the background noise is suppressed, and the reliability increased.

By combining FRET and our technology, our immunoassays are an analytical method which can be used for the detection and the quantification of specific proteins, biomolecules, peptides, cytokines, hormones or antibodies, present in serum, plasma or cell culture supernatant. This method is fast, wash-free, eliminates background fluorescence and economical. Moreover, with Bright-Dtech^TM, the limit of detection and the dynamic range are improved.

You can discover our range of ready-to-use TR-FRET kits for the following biomarkers:

We are currently developing new tests in our R&D center, visit our website regularly to discover them.