NoW-Dtech™ – TR-FRET Assay Kits
NoW-Dtech™ TR-FRET Assay Kits provide a simple and sensitive solution for the detection and quantification of specific biomarkers or biological molecules in complex samples. Based on Time-resolved Fluorescence Resonance Energy Transfer (TR-FRET), these homogeneous assays combine the low background of time-resolved fluorescence (TRF) with the proximity-based detection of FRET.
In TR-FRET, energy transfer occurs when a fluorescent donor and acceptor are brought into close proximity. The efficiency of this transfer depends on the distance between the two fluorophores and the spectral overlap between donor emission and acceptor absorption.
In NoW-Dtech™ TR-FRET assays, Bright-Dtech™ nanoparticles are used as the fluorescent donor. Their long-lived lanthanide emission, occurring on the millisecond timescale, enables a time delay between excitation and fluorescence measurement. This time-resolved detection significantly reduces short-lived background fluorescence from the sample and assay components, improving signal-to-background performance.
By combining Bright-Dtech™ technology with TR-FRET, NoW-Dtech™ TR-FRET Assay Kits enable sensitive and quantitative detection of proteins, peptides, cytokines, hormones, antibodies, and other biomolecules in samples such as serum, plasma, and cell culture supernatants.
Key benefits:
- Wide Dynamic Range: Enables quantification across a broad range of target concentrations.
- High Sensitivity: Time-resolved detection reduces short-lived background fluorescence, supporting sensitive detection of low-abundance targets.
- Homogeneous and No-Wash: Designed for simple assay workflows without separation or washing steps.
- Ease of Use: Ready-to-use kits with clear, step-by-step protocols for straightforward assay implementation.
Applications:
NoW-Dtech™ TR-FRET Assay Kits can be used in a wide range of research applications, including:
- Biomarker and molecule detection
- Biomarker identification and validation
- Drug discovery and development
- Protein-protein interaction studies
- Signal transduction studies
Discover our range of ready-to-use NoW-Dtech™ TR-FRET Assay Kits:
Human IgG
Ref: FRET-hIgG
- LOD: 0.2 ng/mL
- Assay range: 0.98 – 1 000 ng/mL
- Incubation time: 1.5 h
- Specificity: Human IgG
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Mouse IgG
Ref: FRET-mIgG
- LOD: 2 ng/mL
- Assay range: 0.98 – 1 000 ng/mL
- Incubation time: 1.5 h
- Specificity: Mouse IgG
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Human IgM
Ref: FRET-hIgM
- LOD: 0.5 ng/mL
- Assay range: 0.98 – 500 ng/mL
- Incubation time: 1.5 h
- Specificity: Human IgM
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Human CRP
Ref: FRET-hCRP
- LOD: 110 pg/mL
- Assay range: 600 – 100 000 pg/mL
- Incubation time: 1.5 h
- Specificity: Human CRP
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Anti-HER2 antibody (Monkey)
Ref: FRET-mkTrastu
- LOD: 75 pg/mL
- Assay range: 11.7 – 3 000 ng/mL
- Incubation time: 1.5 h
- Specificity: Anti-HER2 antibodies (Monkey)
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Control Kit 545/520 nm
Ref: FRET-CKgreen
The NoW-Dtech™ Control Kit 545/525 nm is designed to assess the compatibility of your TR-FRET reader (green parameters) with Poly-Dtech’s lanthanide-based technology.
Each kit is provided in a 384-well plate format
Control Kit 616/665 nm
Ref: FRET-CKred
The NoW-Dtech™ Control Kit 616/665 nm is designed to assess the compatibility of your TR-FRET reader (red parameters) with Poly-Dtech’s lanthanide-based technology.
Each kit is provided in a 384-well plate format
We are currently developing new tests in our R&D center, visit our website regularly to discover them.