NoW-Dtech™ – TR-FRET Assay Kits

NoW-Dtech™ TR-FRET Assay Kits are a cutting-edge solution designed for accurate and reliable detection of target biomarkers or molecules in biological samples. Indeed, TR-FRET is the combination of TRF (Time-resolved fluorescence) to get a low background signal and FRET (Förster resonance energy transfer) which allows the homogeneous assays.

The transfer of energy resulting from an interaction between two light-sensitive molecules (an acceptor and a donor) because the distance between both entities is small. The transfer is also possible thanks to the compatibility of the wavelengths of the two chromophores. Based on Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology, our NoW-Dtech TR-FRET Assay Kits offer exceptional sensitivity and specificity for your research needs.

Using our Bright-Dtech™ as a donor, background noise will be supressed. Indeed, our lanthanides-based fluorescent nanoparticles have the particularity of having a long lifetime as they emit long fluorescence (several milliseconds). Thus, it is possible to have a delay between the excitation/emission of the nanoparticle and the reading. Thanks to this delay, the background noise is suppressed, and the reliability increased.

TR-FRET Principle with Bright-Dtech technology

By combining FRET and our technology, our NoW-Dtech™ TR-FRET Assay Kits are an analytical method which can be used for the detection and the quantification of specific proteins, biomolecules, peptides, cytokines, hormones or antibodies, present in serum, plasma or cell culture supernatant. They present unique characteristics:

  • Wide Dynamic Range: With its broad dynamic range, NoW-Dtech™ allow the quantification of both high and low concentrations of target molecules, ensuring accurate results across a wide range of sample concentrations
  • High Performance: NoW-Dtech supress background noise and provide reliable data for your assays
  • Ease of Use: NoW-Dtech are ready-to-use with clear step-by-step instructions, making it suitable for both experienced researchers and newcomers to TR-FRET Assay Kits

TR-FRET Assay Kits find extensive application in various research areas, including:

  • Biomarker identification and validation
  • Drug discovery and development
  • Protein-protein interactions
  • Signal transduction studies

You can discover our range of ready-to-use TR-FRET kits for the following biomarkers:

Multipipettes and plate for Human IgG TR-FRET Assay Kits

Human IgG

Ref: FRET-hIgG

  • LOD: 12 pg/mL
  • Assay range: 0.05 – 1200 pg/mL
  • Incubation time: 2h30
  • Specificity: Human IgG

Available in: 96, 480, 960, 9600, 19200 tests

Multipipettes and plate for PSA TR-FRET Assay Kits

Trastuzumab (Monkey)

Ref: FRET-mTrastu

  • LOD: 75 pg/mL
  • Assay range: 11.7 – 3000 ng/mL
  • Incubation time: 1h30
  • Specificity: Trastuzumab/Herceptin®

Available in: 96, 480, 960, 9600, 19200 tests

Multipipettes and plate for PSA TR-FRET Assay Kits

Human Prostate-Specific Antigen (PSA)

Ref: FRET-PSA

  • Detection limit:
  • Assay range:
  • Incubation time:
  • Specificity:

Available in: 96, 480, 960, 9600, 19200 tests

We are currently developing new tests in our R&D center, visit our website regularly to discover them.