NoW-Dtech™ – TR-FRET Assay Kit
Anti-HER2 antibody (Monkey)

Description

Accurate detection and quantification of anti-HER2 antibodies in non-human primate (monkey) samples are important for a wide range of research applications, including biomarker studies, immunological research, and biopharmaceutical development. TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) provides a sensitive and homogeneous approach for quantitative protein detection based on the proximity of fluorescent donor and acceptor molecules.

The NoW-Dtech™ Anti-HER2 Antibody (Monkey) Kit enables the quantitative detection of anti-HER2 antibodies in monkey serum or plasma using a fast, homogeneous, and wash-free mix-and-read assay format.

The assay uses Bright-Dtech™ fluorescent lanthanide nanoparticles as long-lived fluorescent donors. Their time-resolved emission helps reduce short-lived background fluorescence, supporting sensitive and reliable detection in complex biological samples.

TR-FRET Assay Principle

The NoW-Dtech™ Anti-HER2 Antibody (Monkey) assay uses HER2 antigen coupled to Bright-Dtech™ lanthanide nanoparticles, which act as the fluorescent donor, together with a fluorescent acceptor-labelled antibody that recognizes the anti-HER2 antibodies present in the sample.

When anti-HER2 antibodies are present in the sample, they bind to the HER2 antigen displayed on the Bright-Dtech™ nanoparticles. The acceptor-labelled antibody then binds to the anti-HER2 antibodies, bringing the donor and acceptor into close proximity and enabling fluorescence resonance energy transfer (FRET) from the donor to the acceptor.

Following excitation, the resulting time-resolved fluorescence signal is measured using a compatible TR-FRET plate reader. The signal increases with the amount of anti-HER2 antibodies present in the sample, enabling their quantitative detection.

Anti-HER2 antibody (Monkey) Kit: Protocol

The NoW-Dtech™ Anti-HER2 Antibody (Monkey) Kit is designed for a simple mix-and-read workflow. After addition of the sample and detection reagents, the assay requires a 1.5 h incubation step, followed by fluorescence measurement. No washing or separation steps are required.

 

Please refer to the data sheet for detailed instructions and recommended assay conditions before use.

Anti-HER2 antibody (Monkey) Kit: Validation

Anti-HER2 antibody (Monkey) Kit: Required materials but not supplied

• Vials
• Precision single- and multichannel pipettes with disposable tips
• Adhesive sealing film or plate covers
• Plate reader with TR-FRET option

 

Anti-HER2 antibody (Monkey) Kit: Detection system

For optimal assay performance, fluorescence should be measured using a compatible time-resolved fluorescence plate reader with TR-FRET capability. Instrument settings may vary depending on the reader used.

Contact our team if you need assistance selecting a suitable detection system for your NoW-Dtech™ assay.

 

 

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team.

 

Scientific Publications

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors.
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles.
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069