FLISA-Dtech™ – The new generation of ELISA Assay Kits

FLISA-Dtech are a cutting-edge solution designed for accurate and reliable detection of target molecules in biological samples. TR-FLISA is a variant of the ELISA technique which replaces the enzymatic reaction by fluorescence signal.

The objective of TR-FLISA is to improve the detection and the quantification of specific proteins. Poly-Dtech with its Bright-Dtech technology, offers its own TR-FLISA Assay Kits applicable in different approaches such as Sandwich FLISA and Indirect FLISA. FLISA-Dtechalso offer exceptional sensitivity and specificity for your research needs.

Bright-Dtech™ technology allows a long photo-stability and a low background noise which decrease the limit of detection.

FLISA (ELISA assay kits by fluorescence) principle with Bright-Dtech

FLISA-Dtech are used as traditional ELISA Assay Kits. Indeed, the microplate is already coated with the captured antibody or the protein. Then, the following steps are similar with washing, sample addition and incubation. Finally, the only difference is the detection step where nanoparticle is added for the reading.

Benefits of ELISA Assay Kits by fluorescence

Decreased limit of detection
Low background noise
Long photostability
Reduced working time (from 4h to 2h)

Through TR-FLISA technology, FLISA-Dtech are an analytical method which can be used for the detection and the quantification of specific proteins, biomolecules, peptides, cytokines, hormones or antibodies, present in serum, plasma or cell culture supernatant.

  • Wide Dynamic Range: With its broad dynamic range, FLISA-Dtech allow the quantification of both high and low concentrations of target molecules, ensuring accurate results across a wide range of sample concentrations.
  • High Performance: FLISA-Dtech remove background noise and provide reliable data for your assays. They also provide high sensitivity with a low LOD and an exceptional lifetime emission without photobleaching.
  • Ease of Use: FLISA-Dtech are ready-to-use with clear step-by-step instructions, making it suitable for both experienced researchers and newcomers to TR-FLISA Assay Kits. By replacing the enzymatic reaction by fluorescence signal, the working will be reduced from 4h to 2h.

These variants of ELISA Assay Kits find extensive applications in various research areas, including:

  • Biomarker or molecules detection
  • Biomarker identification and validation
  • Drug discovery
  • Protein-protein interactions
  • Signal transduction studies

You can discover our range of ready-to-use TR-FLISA kits for the following biomarkers:

Multipippettes and plates for TR-FLISA or ELISA assay kit by fluorescence

SARS-CoV-2 N protein human IgG

Ref: FLI-hCovid19NIgG

  • LOD: 68 pg/mL
  • Assay range: 0.02 – 100 ng/mL
  • Incubation time: 2h30
  • Specificity: Anti-N protein (SARS-CoV-2) IgG from human samples

Available in: 96, 480, 960, 9600, 19200 tests

Multipippettes and plates for TR-FLISA or ELISA assay kits by fluorescence

Trastuzumab (Monkey)


  • Detection limit: 59 pg/mL
  • Assay range: 0.19 – 3000 pg/mL
  • Incubation time: 2h30
  • Specificity: Trastuzumab from monkey samples

Available in: 96, 480, 960, 9600, 19200 tests

We are currently developing new tests in our R&D center, visit our website regularly to discover them.