FLISA-Dtech™ – SARS-CoV-2 N protein Human IgG Kit (Indirect)
The SARS-Cov-2 is the virus responsible for the Covid-19 pandemic which is member of the coronavirus family. Coronaviruses are enveloped viruses with an RNA-positive genome and a helically symmetrical nucleocapsid. The nucleocapsid protein (N protein) is the most common protein in coronaviruses.
Our FLISA-Dtech™ SARS-CoV-2 (IgG) Kit allows the quantitative determination of human IgG antibody against SARS-CoV-2 N protein present in serum and plasma from human using a fast, no photobleaching and cost-effective method. Indeed, TR-FLISA is a variant of the ELISA technique which replaces the enzymatic reaction by fluorescence signal and provides exceptional sensitivity and robustness, allowing the detection and quantification of various biomolecular interactions. FLISA-Dtech™ includes Bright-Dtech™ technology based on lanthanides which provide exceptional detection due to its high specificity and sensitivity.
TR-FLISA Assay Principle
In Bright-Dtech™ TR-FLISA method, detection/secondary antibodies are conjugated to fluorescent nanoparticles. A measure of the fluorescent intensity is detected by exposing the microplate at a particular wavelength using time-resolved fluorescent reader. Indirect FLISA is used for the detection of an antibody against to an antigen immobilized to the surface of the multi-well plate. In indirect FLISA, a Bright-Dtech-conjugated secondary antibody against the host species of the primary antibody is used for detection.
SARS-CoV-2 N protein (hIgG) Kit: Protocole
Read the data sheet in detail before use
SARS-CoV-2 N protein (hIgG) Kit: Validation
SARS-CoV-2 N protein (hIgG) Kit: Required materials
- Deionized or disilled water
- Precision pipettes calibrated to deliver 5-1000µL
- Multi channel pipettes
- Adhesive sealing film or plates
- Automatic plate washer
For all technical support, commercial enquiries or further information, contact the Poly-Dtech team
|Dimensions||14 × 15 × 11 cm|
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