Custom Multiplex Assay Development

Poly-Dtech offers custom multiplex assay development that is subjected to a thorough and rigorous process to guarantee excellent quality and reproducibility. Custom multiplex assay develoment by Poly-Dtech allows the detection of various biomarkers simultaneously in the same assay through TR-FRET method.

Indeed, Poly-Dtech can develop your own multiplex assay or convert your ELISA assay into a mix and read test for a faster and more accurate detection of biomarkers simultaneously.

We offer the research, development and the validation of a custom multiplex assay development that fit with your needs.

Custom Multiplex Development (Preliminary Research)

Preliminary Research

The first step of the development process is to define the specifications of the project and the best strategy to be applied. One or more recommendations could be proposed in function of:

  • Type of test: TR-FRET
  • Targeted analyte: antibody, protein, biomolecules, peptide, small molecule, etc.
  • Types of readouts: Quantitative, Semi-quantitative, qualitative.
  • Matrix: serum, plasma, urine, saliva, water, etc.
  • Evaluation and selection of the best components: membrane, strip, buffer, antigens, bioreceptor (proteins, antibodies, or DNA).
Custom Multiplex Development (Optimization)


Our multiplexing solution is based on TR-FRET which is a fast, no-wash and low-interference technique. TR-FRET immunoassay is an analytical method which can be used for the detection and quantification of biomarker present in serum, plasma and cell supernatant. Our Bright-Dtech™ fluorescent nanoparticles bring a more sensitive and stable approach. In Bright-Dtech™ TR-FRET method, we design our nanoparticles and accpetors to detect biomarkers with high specificity and sensitivy and at a low price.

Custom Multiplex Development (Validation)


Validating the method ensures results are reproducible, reliable, robust, and accurate. Validation of the analytical method will be subjected to the following experimental checks:

  • Specificity (the method must differentiate the targeted analyte from all other matrix components)
  • Linearity (range, LLOQ)
  • Sensitivity (LOD)
  • Accuracy (spiking recovery test)
  • Precision (repeatability = intra assay, inter assay; reproducibility = inter laboratory assay, or on different days or different plates) Robustness (defined as the ability to remain unaffected by small variations in method parameters. These parameters could be incubation temperature, temperature of used components or varied incubation time due to time delay during pipetting).

Since 2023, Poly-Dtech is CIR (Research Tax Credit) approved, allowing a tax reduction of 30% for companies that call on our R&D services for the next three years. Find here the approval

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