Multi-Dtech™ – Multiplex TR-FRET Assay Kits
Multi-Dtech™ Multiplex TR-FRET Assay Kits provide a simple and sensitive solution for the simultaneous detection and quantification of multiple biomarkers or biological molecules in a single assay. Based on TR-FRET technology, Multi-Dtech™ combines time-resolved fluorescence with multiplex detection to increase assay throughput while maintaining a homogeneous, no-wash workflow.
Multiplexing enables several targets to be measured simultaneously by using fluorescent labels with distinct emission profiles and an appropriate detection system. Bright-Dtech™ lanthanide nanoparticles provide long-lived and spectrally distinct fluorescence, making them well suited for multiplex TR-FRET assays.
TR-FRET immunoassays enable the sensitive detection and quantification of biomarkers and other biological molecules in complex samples such as serum, plasma, and cell culture supernatants. In the Bright-Dtech™ TR-FRET format, fluorescent lanthanide nanoparticles are used as long-lived donors and combined with specific acceptors to generate a fluorescence signal upon molecular proximity. This approach enables sensitive and specific detection while supporting multiplex assay development using nanoparticles with distinct emission profiles.
By combining Bright-Dtech™ technology with multiplex TR-FRET, Multi-Dtech™ Multiplex TR-FRET Assay Kits enable the simultaneous detection and quantification of proteins, peptides, cytokines, hormones, antibodies, and other biomolecules in samples such as serum, plasma, and cell culture supernatants.
Key benefits:
- Multiplex Detection: Detect multiple biomarkers simultaneously in a single assay.
- Wide Dynamic Range: Enables quantification across a broad range of target concentrations.
- High Sensitivity: Time-resolved detection reduces short-lived background fluorescence, supporting sensitive detection of low-abundance targets.
- Ease of Use: Ready-to-use kits with clear, step-by-step protocols for straightforward assay implementation.
- Fast: Based on TR-FRET technique, Multi-Dtech™ don’t requiere washing steps and thus reduce working time.
Applications:
Multi-Dtech™ Multiplex TR-FRET Assay Kits can be used in a wide range of research applications, including:
- Biomarker and molecule detection
- Biomarker identification and validation
- Drug discovery and development
- Protein-protein interaction studies
- Signal transduction studies
Discover our range of ready-to-use Multiplex TR-FRET Assay Kits:
Human IgG / IgM
Reference: Multi-hIgG/hIgM
- Human IgG / IgM detection limit: 1.6 / 6 ng/mL
- Assay range: 1 – 500 ng/mL
- Incubation time: 1.5 h
- Specificity: Human IgG / Human IgM
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Mouse IgG / IgM
Reference: Multi-mIgG/mIgM
- Mouse IgG / IgM detection limit: 0.72 / 2 ng/mL
- Assay range: 1 – 1 000 ng/mL
- Incubation time: 1.5 h
- Specificity: Mouse IgG / Mouse IgM
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Control Kit 545/520 nm
Ref: FRET-CKgreen
The NoW-Dtech™ Control Kit 545/525 nm is designed to assess the compatibility of your TR-FRET reader (green parameters) with Poly-Dtech’s lanthanide-based technology.
Each kit is provided in a 384-well plate format
Control Kit 616/665 nm
Ref: FRET-CKred
The NoW-Dtech™ Control Kit 616/665 nm is designed to assess the compatibility of your TR-FRET reader (red parameters) with Poly-Dtech’s lanthanide-based technology.
Each kit is provided in a 384-well plate format
We are currently developing new tests in our R&D center, visit our website regularly to discover them.