Multi-Dtech™
Multiplex TR-FRET Assay Kit
Mouse IgG/IgM

Description

Accurate detection and quantification of mouse IgG and IgM are important for a wide range of research applications, including biomarker studies, immunological research, and biopharmaceutical development. Multi-Dtech™ provides a sensitive and homogeneous approach for the simultaneous quantitative detection of multiple biomarkers based on TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer).

The Multi-Dtech™ Mouse IgG/IgM Kit enables the simultaneous quantitative detection of mouse IgG and IgM in serum, plasma, and cell culture supernatants using a fast, homogeneous, and wash-free mix-and-read assay format.

The assay uses Bright-Dtech™ fluorescent lanthanide nanoparticles as long-lived fluorescent donors, with distinct emission profiles for multiplex detection. Their time-resolved emission helps reduce short-lived background fluorescence, supporting sensitive and reliable detection in complex biological samples.

Assay Principle

The Multi-Dtech™ Mouse IgG/IgM assay uses two antibody pairs, with one pair specifically targeting mouse IgG and the other targeting mouse IgM. For each target, one antibody is coupled to a Bright-Dtech™ lanthanide nanoparticle donor, while the second antibody is coupled to a fluorescent acceptor.

When the antibody pairs bind to their respective targets, the donor and acceptor are brought into close proximity, enabling fluorescence resonance energy transfer (FRET). Each donor–acceptor pair generates a distinct fluorescence signal corresponding to either mouse IgG or IgM. The two signals are simultaneously measured using a compatible TR-FRET plate reader, enabling quantitative analysis of both targets in a single reaction.

Mouse IgM/IgG Kit: Protocol

The Multi-Dtech™ Mouse IgG/IgM Kit is designed for a simple mix-and-read workflow. After addition of the sample and detection reagents, the assay requires a 1.5 h incubation step, followed by fluorescence measurement. No washing or separation steps are required.

 

Please refer to the data sheet for detailed instructions and recommended assay conditions before use.

 

Mouse IgM/IgG Kit: Validation

Mouse IgG/IgM Kit: Required materials but not supplied

• Vials
• Precision single- and multichannel pipettes with disposable tips
• Adhesive sealing film or plate covers
• Plate reader with TR-FRET option

 

Mouse IgG/IgM Kit: Detection system

For optimal assay performance, fluorescence should be measured using a compatible time-resolved fluorescence plate reader with TR-FRET capability. Instrument settings may vary depending on the reader used.

Contact our team if you need assistance selecting a suitable detection system for your NoW-Dtech™ assay.

 

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team.

 

Scientific Publications

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors.
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles.
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069