NoW-Dtech™ – Control Kit
Green – 545/520 nm

Description

The NoW-Dtech™ Control Kit 545/520 nm (Green) is designed to assess the compatibility of your TR-FRET reader with Poly-Dtech’s lanthanide-based technology.

It contains Bright-Dtech™ donor nanoparticles (Green) and an Acceptor that generates a TR-FRET signal upon binding, enabling you to optimize your reader parameters for maximum sensitivity and optimal signal-to-noise ratio.

 

Control kit: Protocol

The NoW-Dtech™ Control (Green) Kit is designed for a simple mix-and-read workflow. After addition of the working standard and detection reagent, the assay requires a 1.5 h incubation step, followed by fluorescence measurement. No washing or separation steps are required.

Please refer to the data sheet for detailed instructions and recommended assay conditions before use.

Control kit: Validation

 

Control Kit: Required materials but not supplied

• Vials
• Precision single- and multichannel pipettes with disposable tips
• Adhesive sealing film or plate covers
• Plate reader with TR-FRET option

 

Control Kit: Detection system

For optimal assay performance, fluorescence should be measured using a compatible time-resolved fluorescence plate reader. Instrument settings may vary depending on the reader used.

Contact our team if you need assistance selecting a suitable detection system for your FLISA-Dtech™ assay.

 

 

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team.

 

 

Scientific Publications

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors.
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles.
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069