NoW-Dtech™ – TR-FRET Assay Kit
Human IgM

Description

Exploring protein levels of Human Total IgM and drug discovery requires precise and accurate detection and quantification. For this purpose, TR-FRET has emerged as a powerful and versatile tool in the field, revolutionizing how researchers study molecular interactions and screen potential drug candidates. Harnessing the principles of FRET, TR-FRET assays offer exceptional sensitivity and robustness, enabling the detection and quantification of various biomolecular interactions in a single step (mix-and-read test).

Our NoW-Dtech™ Human IgM Kit allows for the quantitative detection of human IgM present in serum, plasma, or cell culture supernatants using a fast, wash-free, and cost-effective method. NoW-Dtech™ includes Bright-Dtech™ technology based on lanthanides, which provides exceptional detection performance due to its high specificity and sensitivity.

TR-FRET Assay Principle

The Bright-Dtech™ TR-FRET method employs two antibodies to detect human IgM. The first antibody is conjugated to Bright-Dtech™ (the donor), and the second is coupled with fluorescent molecules (the acceptor). When both antibodies bind to their target, the fluorescent energy from the donor is transferred to the acceptor. This transfer is then detected using a time-resolved fluorescence reader, yielding a specific and quantitative result.

Human IgM Kit: Protocol

As shown above, the Human IgM Kit requires only a single 2h30 incubation step after the addition of the detection reagents. Read the data sheet in detail before use.

Human IgM Kit: Validation

Human IgM Kit: Required materials but not supplied

• Vials
• Precision single and multichannel pipettes with disposable tips
• Adhesive sealing film or plates

 

Choosing the right plate reader allows to get the best possible results. NoW-Dtech™ products are compatible with TR-FRET plate readers.

 

 

 

 

 

 

 

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team