NoW-Dtech™ – TR-FRET Assay Kit
Human CRP

Description

Exploring Human CRP Levels with Precision and Accuracy

Exploring the levels of human C-reactive protein (CRP) and its role in various inflammatory conditions requires precise and accurate detection and quantification. For this purpose, TR-FRET has emerged as a powerful and versatile tool in the field, revolutionizing how researchers study biomolecular interactions and detect key biomarkers. Harnessing the principles of FRET, TR-FRET assays offer exceptional sensitivity and robustness, enabling the detection and quantification of molecular interactions in a single-step, mix-and-read format.

Our NoW-Dtech™ – Human CRP TR-FRET assay kit allows for the quantitative detection of the CRP biomarker in serum, using a fast, wash-free, and cost-effective method. NoW-Dtech™ incorporates Bright-Dtech™ technology, based on lanthanides, providing outstanding specificity and sensitivity for reliable and precise detection.

 

TR-FRET Assay Principle

The NoW-Dtech™ Human CRP assay uses two antibodies directed against human CRP. One antibody is coupled to Bright-Dtech™ lanthanide nanoparticles, which act as the fluorescent donor, while the second antibody is coupled to a fluorescent acceptor.

When both antibodies bind to human CRP, the donor and acceptor are brought into close proximity, enabling fluorescence resonance energy transfer (FRET) from the donor to the acceptor. Following excitation, the resulting time-resolved fluorescence signal is measured using a compatible TR-FRET plate reader. The measured signal is proportional to the amount of human CRP present in the sample, enabling quantitative analysis.

Human CRP assay kit: Protocol

The NoW-Dtech™ Human CRP Kit is designed for a simple mix-and-read workflow. After addition of the sample and detection reagents, the assay requires a 1.5 h incubation step, followed by fluorescence measurement. No washing or separation steps are required.

 

Please refer to the data sheet for detailed instructions and recommended assay conditions before use.

 

Human CRP assay kit: Validation

Human CRP Kit: Required materials but not supplied

• Vials
• Precision single- and multichannel pipettes with disposable tips
• Adhesive sealing film or plate covers
• Plate reader with TR-FRET option

 

Human CRP Kit: Detection system

For optimal assay performance, fluorescence should be measured using a compatible time-resolved fluorescence plate reader with TR-FRET capability. Instrument settings may vary depending on the reader used.

Contact our team if you need assistance selecting a suitable detection system for your NoW-Dtech™ assay.

 

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team.

 

Scientific Publications

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors.
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles.
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069