NoW-Dtech™ – Trastuzumab (Monkey) Kit

Description

Exploring levels of Trastuzumab (Herceptin^®) and drug discovery require precise and accurate detection and quantification. For this purpose, TR-FRET has emerged as a powerful and versatile tool in the field, revolutionizing the way researchers study molecular interactions and screen potential drug candidates. By harnessing the principles of FRET, TR-FRET assays provide exceptional sensitivity and robustness, allowing for the detection and quantification of various biomolecular interactions in one step (mix and read).

Our NoW-Dtech™ Trastuzumab (Monkey) Kit allows the quantitative detection free functional Trastuzumab (Herceptin^®) present in serum and plasma from monkeys using a fast, wash-free and cost-effective method. NoW-Dtech™ includes Bright-Dtech™ technology based on lanthanides which provide exceptional detection due to its high specificity and sensitivity.

TR-FRET Assay Principle

Bright-Dtech™ TR-FRET method is based on two antibodies to detect Trastuzumab (Herceptin^®). Indeed, the HER2 is conjugated to Brigh-Dtech™(donor) and the second one is coupled with fluorescent molecules (acceptor). If both antibodies recognize the target, the fluorescent intensity of the donor is transferred to the acceptor and the signal is detected using timeresolved fluorescence reader to obtain a specific and quantitative result.

Tastuzumab (Monkey) Kit: Protocol

 

As shown bellow, the Trastuzumab (Monkey) Kit requires only one incubation step of 1h30 after the addition of detection reagents. Read the data sheet in detail before use.

Trastuzumab (Monkey) Kit: Validation

Trastuzumab (Monkey) Kit: Required materials

• Vials
• Precision single and multichannel pipettes with disposable tips
• Adhesive sealing film or plates

Additional information

Data Sheet

 

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team