FLISA-Dtech™ – Trastuzumab (Monkey) Kit

TR-FLISA Kits benefits

SKU: FLI-MonTRASTU Category: Tags: ,


Trastuzumab is a fully humanized (IgG1 kappa) monoclonal antibody selective for the domain IV of the extracellular domain of the human epidermal growth factor receptor type 2 (HER2). Trastuzumab (trade name Herceptin®) is indicated for treating cancers overexpressing HER2 such as breast cancer, stomach cancer and gastroesophageal junction cancer.

Our FLISA-Dtech™ Trastuzumab (Monkey) Kit allows the quantitative determination of free functional Trastuzumab present in serum and plasma from monkeys using a fast, no photobleaching and cost-effective method. Indeed, TR-FLISA is a variant of the ELISA technique which replaces the enzymatic reaction by fluorescence signal and provides exceptional sensitivity and robustness, allowing the detection and quantification of various biomolecular interactions. FLISA-Dtech™ includes Bright-Dtech™ technology based on lanthanides which provide exceptional detection due to its high specificity and sensitivity.


TR-FLISA Assay Principle

In Bright-Dtech™ TR-FLISA method, detection/secondary antibodies are conjugated to fluorescent nanoparticles. A measurement of the fluorescent intensity is detected by exposing the microplate at a particular wavelength using time-resolved fluorescent reader. Indirect FLISA is used for the detection of an antibody against to an antigen immobilized to the surface of the multi-well plate. In indirect FLISA, a Bright-Dtech-conjugated secondary antibody against the host species of the primary antibody is used for detection.

Indirect FLISA diagram for Trastuzumab kit


Trastuzumab (Monkey) Kit: Protocol

Protocol overview of TR-FLISA protocol for Trastuzumab kit


Read the data sheet in detail before use


Trastuzumab (Monkey) Kit: Validation


Typical standard curve trastuzumab

Validation and settings for trastuzumab kit

Trastuzumab (Monkey) Kit: Supplemental required materials

  • Deionized or distilled water
  • Precision pipettes calibrated to deliver 5-1000 µL
  • Multi channel pipettes
  • Adhesive sealing film or plates
  • Automatic plate washer

Additional information

Data Sheet


For all technical support, commercial enquiries or further information, contact the Poly-Dtech team

Additional information

Weight 0.7 kg
Dimensions 14 × 15 × 11 cm

96 tests, 480 tests, 960 tests, 4800 tests, 9600 tests, 19200 tests