NoW-Dtech™ – Human IgG Kit

Description

Exploring protein levels of Human Total IgG and drug discovery require precise and accurate detection and quantification. For this purpose, TR-FRET has emerged as a powerful and versatile tool in the field, revolutionizing how researchers study molecular interactions and screen potential drug candidates. Harnessing the principles of FRET, TR-FRET assays offer exceptional sensitivity and robustness, enabling the detection and quantification of various biomolecular interactions in a single step (mix-and-read test).

Our NoW-Dtech™ Human IgG Kit allows for the quantitative detection of the human IgG biomarker present in serum, plasma, or cell supernatants using a fast, wash-free and cost-effective method. NoW-Dtech™ includes Bright-Dtech™ technology based on lanthanides which provides exceptional detection due to its high specificity and sensitivity.

TR-FRET Assay Principle

The Bright-Dtech™ TR-FRET method employs two antibodies to detect human IgG. The first antibody is conjugated to Bright-Dtech™(the donor), and the second is coupled with fluorescent molecules (the acceptor). When both antibodies bind to their target, the fluorescent intensity from the donor is transferred to the acceptor. This transfer is then detected using a time-resolved fluorescence reader, yielding a specific and quantitative result.

Human IgG Kit: Protocol

 

As shown above, the Human IgG Kit requires only a single 2h30 incubation step after the addition of the detection reagents. Read the data sheet in detail before use.

Human IgG Kit: Validation

Human IgG Kit: Required materials

• Vials
• Precision single and multichannel pipettes with disposable tips
• Adhesive sealing film or plates

 

Choosing the right plate reader allows to get the best possible results. NoW-Dtech™ products are compatible with TR-FRET plate readers.

 

 

 

 

 

 

 

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team