NoW-Dtech™ – TR-FRET Assay Kit
Human CRP

Description

Exploring Human CRP Levels with Precision and Accuracy

Exploring the levels of human C-reactive protein (CRP) and its role in various inflammatory conditions requires precise and accurate detection and quantification. For this purpose, TR-FRET has emerged as a powerful and versatile tool in the field, revolutionizing how researchers study biomolecular interactions and detect key biomarkers. Harnessing the principles of FRET, TR-FRET assays offer exceptional sensitivity and robustness, enabling the detection and quantification of molecular interactions in a single-step, mix-and-read format.

Our NoW-Dtech™ – Human CRP TR-FRET assay kit allows for the quantitative detection of the CRP biomarker in serum, using a fast, wash-free, and cost-effective method. NoW-Dtech™ incorporates Bright-Dtech™ technology, based on lanthanides, providing outstanding specificity and sensitivity for reliable and precise detection.

TR-FRET Assay Principle

The Bright-Dtech™ TR-FRET method employs two antibodies to detect human CRP. The first antibody is conjugated to Bright-Dtech™(the donor), and the second is coupled with fluorescent molecules (the acceptor). When both antibodies bind to their target, the fluorescent intensity from the donor is transferred to the acceptor. This transfer is then detected using a time-resolved fluorescence reader, yielding a specific and quantitative result.

Human CRP assay kit: Protocol

 

As shown above, the Human CRP Kit requires only a single 1h30 incubation step after the addition of the detection reagents. Read the data sheet in detail before use.

Human CRP assay kit: Validation

Human CRP assay Kit: Required materials

• Vials
• Precision single and multichannel pipettes with disposable tips
• Adhesive sealing film or plates

Choosing the right plate reader allows to get the best possible results. NoW-Dtech™ products are compatible with TR-FRET plate readers.

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team

Additional documents

• Data Sheet
• Security Sheet

Publications and references

Ultra-bright lanthanide nanoparticles
Terbium-doped LaF3 nanoparticles, surface-functionalized by photon-harvesting antenna ligands. Surface capping with antenna ligands leads to ultrabright nanoparticles, with the typical green luminescence signature of Tb atoms and very long excited-state lifetimes
Joan Goetz, Aline Nonat, Abdoulaye Diallo, Mohamadou Sy, Ildan Sera, Alexandre Lecointre, Christophe Lefevre, Chi Fai Chan, Ka-Leung Wong, Loïc J Charbonnière
Chempluschem. 2016 Jun;81(6):497.
doi: 10.1002/cplu.201600117

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007