FLISA-Dtech™ – FLISA Assay Kit
Human – SARS-CoV-2 N protein IgG (Indirect)

Description

The SARS-Cov-2 is the virus responsible for the Covid-19 pandemic which is member of the coronavirus family. Coronaviruses are enveloped viruses with an RNA-positive genome and a helically symmetrical nucleocapsid. The nucleocapsid protein (N protein) is the most common protein in coronaviruses.

Our FLISA-Dtech™ SARS-CoV-2 (IgG) Kit allows the quantitative determination of human IgG antibody against SARS-CoV-2 N protein present in serum and plasma from human using a fast, no photobleaching and cost-effective method. Indeed, TR-FLISA is a variant of the ELISA technique which replaces the enzymatic reaction by fluorescence signal and provides exceptional sensitivity and robustness, allowing the detection and quantification of various biomolecular interactions. FLISA-Dtech™ includes Bright-Dtech™ technology based on lanthanides which provide exceptional detection due to its high specificity and sensitivity.

TR-FLISA Assay Principle

In Bright-Dtech™ TR-FLISA method, detection/secondary antibodies are conjugated to fluorescent nanoparticles. A measure of the fluorescent intensity is detected by exposing the microplate at a particular wavelength using time-resolved fluorescent reader. Indirect FLISA is used for the detection of an antibody against to an antigen immobilized to the surface of the multi-well plate. In indirect FLISA, a Bright-Dtech-conjugated secondary antibody against the host species of the primary antibody is used for detection.

 

 

SARS-CoV-2 N protein (hIgG) Kit: Protocole

 

Read the data sheet in detail before use

SARS-CoV-2 N protein (hIgG) Kit: Validation

SARS-CoV-2 N protein (hIgG) Kit: Required materials

• Deionized or disilled water
• Precision pipettes calibrated to deliver 5-1000µL
• Multi channel pipettes
• Adhesive sealing film or plates
• Automatic plate washer

Additional information

Data Sheet

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team