Multi-Dtech™
Multiplex TR-FRET Assay Kit
Mouse IgM/IgG

Description

The Multi-Dtech™ kit enables the quantitative and simultaneous detection of mouse IgM and IgG in a single reaction from serum or cell culture supernatant. Based on the TR-FRET (Time-Resolved Förster Resonance Energy Transfer) method, this no-wash assay offers a fast, highly sensitive, and user-friendly protocol with minimal background interference. The kit leverages Bright-Dtech™ technology, delivering exceptional specificity and sensitivity for reliable multiplex biomarker quantification.

Assay Principle

The TR-FRET principle relies on the use of two antibodies targeting the same biomarker:
• One antibody is labeled with Bright-Dtech™ (donor)
• The second is labeled with a fluorescent acceptor

When both antibodies bind to their target (e.g., mouse IgM or IgG), the proximity allows energy transfer from donor to acceptor. The resulting signal is measured using a time-resolved fluorescence reader, ensuring high specificity and quantitative accuracy

Mouse IgM/IgG Kit: Protocol

Mouse IgM/IgG Kit: Validation

Mouse IgM/IgG Kit: Required materials but not supplied

• Vials
• Precision single and multichannel pipettes with disposable tips
• Adhesive sealing film or plates

Choosing the right plate reader allows to get the best possible results. NoW-Dtech™ products are compatible with TR-FRET plate readers.

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team

Additional documents

• Data Sheet
• Security Sheet

Publications and references

Ultra-bright lanthanide nanoparticles
Terbium-doped LaF3 nanoparticles, surface-functionalized by photon-harvesting antenna ligands. Surface capping with antenna ligands leads to ultrabright nanoparticles, with the typical green luminescence signature of Tb atoms and very long excited-state lifetimes
Joan Goetz, Aline Nonat, Abdoulaye Diallo, Mohamadou Sy, Ildan Sera, Alexandre Lecointre, Christophe Lefevre, Chi Fai Chan, Ka-Leung Wong, Loïc J Charbonnière
Chempluschem. 2016 Jun;81(6):497.
doi: 10.1002/cplu.201600117

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007