Link-Dtech™ 545 – Tb (Green) – For LFIA

Description

With Link-Dtech™ – For FLIA, we offer you an innovative solution to achieve higher detection sensitivity in Lateral Flow Assays (LFIA) with easy-to-use protocols. Our fluorescent nano-molecules have been specifically developed for LFIA applications, ensuring optimal signal intensity and rapid detection (Watch our video on Youtube).

Poly-Dtech provides a dedicated conjugation kit, designed for efficient and specific coupling with antibodies, analytes, and other biological components. The nanoparticles in this kit have been engineered for superior performance in LFIA, guaranteeing high stability, strong binding, and enhanced assay sensitivity. Additionally, you can fully customize the nanoparticle surface to meet your specific assay requirements.

 

➡️ Coupling capacity:

With 200 µL of Link-Dtech™, you can typically couple between 15 and 50 µg of antibody, depending on your desired nanoparticle-to-antibody ratio. The optimal amount depends on how densely you wish to coat the nanoparticle surface, and on the size (molecular weight) of the biomolecule.

For example:

• For a standard IgG antibody (~150 kDa), this amount allows coupling of approximately 50 to 200 antibody molecules per nanoparticle, depending on how densely you wish to decorate the surface.
• For a smaller protein, such as streptavidin (~60 kDa), a similar mass (15–50 µg) would correspond to a larger number of protein molecules per nanoparticle, as the molecular weight is lower.
• For larger biomolecules, such as full-length enzymes (~250–300 kDa), fewer molecules will be coupled per nanoparticle for the same total mass.

These values can be adjusted according to your application (signal amplification, steric hindrance, etc.), and custom optimization is available.

If you need assistance with a specific coupling strategy, don’t hesitate to contact us: contact@poly-dtech.com

For more information, please watch the tutorial video “Link-Dtech – How to use?” on YouTube:🔗 https://youtu.be/21HC5tL9Mv4

 

 

Requested Equipment

Additional Documents

• Data Sheet
• Security Sheet

 

Publications and references

Breaking the picomolar barrier in lateral flow assays using Bright-Dtech™ 614 – Europium nanoparticles for enhanced sensitivity,
Enhanced sensitivity in lateral flow assays using Poly-Dtech Europium nanoparticles specifically developed for LFIA applications
Juliette Lajoux, Yulieth D. Banguera-Ordoñez, Amadeo Sena-Torralba, Loïc J. Charbonnière, Mohamadou Sy, Joan Goetz, Ángel Maquieira, Sergi Morais
Microchemical Journal, 2025, Volume 209, 112864
doi: https://doi.org/10.1016/j.microc.2025.112864

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069