Link-Dtech™ 545 – Tb (Green) – For LFIA

Description

With Link-Dtech™ – For LFIA, we offer an innovative solution for the easy development of customized fluorescent nanoparticle conjugates, combining a straightforward conjugation protocol with enhanced detection sensitivity for Lateral Flow Immunoassays (LFIA). Our fluorescent lanthanide nanoparticles are specifically engineered for LFIA applications, delivering high signal intensity and excellent signal-to-background performance for sensitive and reliable detection (Watch our video on YouTube).

Poly-Dtech provides a dedicated conjugation kit designed for the efficient coupling of antibodies, proteins, and other biological molecules to Link-Dtech™ nanoparticles. The conjugation procedure and provided buffers are designed to support stable nanoparticle-biomolecule conjugates for LFIA applications. The nanoparticle surface can be customized with different biomolecules according to your specific assay requirements.

 

➡️ Coupling capacity:

With 200 µL of Link-Dtech™, typically 15–60 µg of antibody can be used for conjugation, depending on the desired antibody-to-nanoparticle ratio.

For a standard IgG antibody (~150 kDa), this corresponds to a typical starting range of approximately 50–200 antibodies per nanoparticle, depending on the selected coupling ratio and the desired surface coverage.

The amount of biomolecule required should be adjusted according to its molecular weight, size, and the desired surface coverage. For example, for the same mass of protein, a smaller protein such as streptavidin (~60 kDa) corresponds to a higher number of molecules than an IgG antibody, whereas larger biomolecules, such as full-length enzymes (~250–300 kDa), correspond to fewer molecules.

The optimal coupling ratio depends on the intended application and may be influenced by factors such as signal intensity, steric accessibility, and nanoparticle surface coverage. Custom optimization is available to identify the most suitable coupling conditions for your assay.

If you need assistance with a specific coupling strategy, please contact us at: contact@poly-dtech.com

For more information on the conjugation procedure, watch our tutorial video “Link-Dtech – How to use?” on YouTube:🔗 https://youtu.be/21HC5tL9Mv4

 

 

Requested Equipment

Additional Documents

• Data Sheet
• Security Sheet

 

Publications and references

Lanthanide nanoparticles as ultra-sensitive luminescent probes for quantitative PSA detection via lateral flow assays.
Enhanced sensitivity in lateral flow assays for quantitative PSA detection using Poly-Dtech lanthanide nanoparticles, specifically developed for ultra-sensitive LFIA applications.
Juliette Lajoux, Mohamadou Sy, Loïc J. Charbonnière, Joan Goetz, Susana Brun
Sensors & Diagnostics, 2025, 5 (1): 76-82.
doi: https://doi.org/10.1039/d5sd00143a

Breaking the picomolar barrier in lateral flow assays using Bright-Dtech™ 614 – Europium nanoparticles for enhanced sensitivity.
Enhanced sensitivity in lateral flow assays using Poly-Dtech Europium nanoparticles specifically developed for LFIA applications.
Juliette Lajoux, Yulieth D. Banguera-Ordoñez, Amadeo Sena-Torralba, Loïc J. Charbonnière, Mohamadou Sy, Joan Goetz, Ángel Maquieira, Sergi Morais
Microchemical Journal, 2025, Volume 209, 112864.
doi: https://doi.org/10.1016/j.microc.2025.112864

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors.
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles.
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069