Bright-Dtech™ 545 – Tb (Green)
– For LFIA –
Coupled to Anti-Human IgG

Description

Optimized for Lateral Flow Immunoassays (LFIA) with Bright-Dtech™

With Bright-Dtech™ – For LFIA, we offer an innovative solution to enhance detection sensitivity in Lateral Flow Immunoassays (LFIA). Our fluorescent lanthanide nanoparticles are specifically engineered for LFIA applications, delivering high signal intensity and excellent signal-to-background performance for sensitive and reliable detection (Watch our video on Youtube).

In LFIA, secondary antibodies play a crucial role in detecting and visualizing a primary antibodies bound to their target analytes. For specific detection of human primary antibodies, a secondary anti-human antibody coupled to Bright-Dtech can be used as detection reagent.

Thanks to their characteristic lanthanide emission spectra, Bright-Dtech™ 545 – Tb (Green) could be combined with other Bright-Dtech™ nanoparticles to enable multiplex detection of multiple biomarkers or proteins on the same LFIA strip, expanding diagnostic capabilities while minimizing spectral overlap.

 

How to Use Bright-Dtech ?

Recommended dilutions may vary depending on the assay format, detection system, and sample type. The following range is provided as a general guideline:

• Dilution range: 1:10 to 1:100
• Optimize according to signal intensity, background, and detection system sensitivity

 

We strongly recommend performing pilot titration experiments to determine the optimal working dilution under your specific assay conditions.

Need help or a custom antibody conjugate? Our team is here to help. Contact us at contact@poly-dtech.com

 

 

Requested Equipment

Additional Documents

• Data Sheet
• Security Sheet

 

Publications and references

Lanthanide nanoparticles as ultra-sensitive luminescent probes for quantitative PSA detection via lateral flow assays.
Enhanced sensitivity in lateral flow assays for quantitative PSA detection using Poly-Dtech lanthanide nanoparticles, specifically developed for ultra-sensitive LFIA applications.
Juliette Lajoux, Mohamadou Sy, Loïc J. Charbonnière, Joan Goetz, Susana Brun
Sensors & Diagnostics, 2025, 5 (1): 76-82.
doi: https://doi.org/10.1039/d5sd00143a

Breaking the picomolar barrier in lateral flow assays using Bright-Dtech™ 614 – Europium nanoparticles for enhanced sensitivity.
Enhanced sensitivity in lateral flow assays using Poly-Dtech Europium nanoparticles specifically developed for LFIA applications.
Juliette Lajoux, Yulieth D. Banguera-Ordoñez, Amadeo Sena-Torralba, Loïc J. Charbonnière, Mohamadou Sy, Joan Goetz, Ángel Maquieira, Sergi Morais
Microchemical Journal, 2025, Volume 209, 112864.
doi: https://doi.org/10.1016/j.microc.2025.112864

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors.
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles.
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069