NoW-Dtech™ – TR-FRET Assay Kit
Anti-HER2 antibody (Monkey)

Description

Exploring levels of Anti-HER2 antibodies and advancing drug discovery require precise and accurate detection and quantification. For this purpose, TR-FRET has emerged as a powerful and versatile tool in the field, revolutionizing how researchers study molecular interactions and screen potential drug candidates. Harnessing the principles of FRET, TR-FRET assays offer exceptional sensitivity and robustness, enabling the detection and quantification of various biomolecular interactions in a single step (mix-and-read test).

Our NoW-Dtech™ – Anti-HER2 antibody (Monkey) Kit allows for the quantitative detection of free functional Anti-HER2 antibodies present in serum and plasma from monkeys using a fast, wash-free and cost-effective method. NoW-Dtech™ includes Bright-Dtech™ technology based on lanthanides, which provides exceptional detection due to its high specificity and sensitivity.

TR-FRET Assay Principle

The Bright-Dtech™ TR-FRET method employs two antibodies to detect Anti-HER2 antibody. The HER2 is conjugated to Brigh-Dtech™ (the donor) and the second is coupled with fluorescent molecules (the acceptor). When both antibodies bind to their target, the fluorescent intensity from the donor is transferred to the acceptor. This transfer is then detected using time-resolved fluorescence reader, yielding a specific and quantitative result.

Anti-HER2 antibody (Monkey) Kit: Protocol

 

As shown above, the Anti-HER2 antibody Kit requires only a single 2h30 incubation step after the addition of detection reagents. Read the data sheet in detail before use.

Anti-HER2 antibody (Monkey) Kit: Validation

Anti-HER2 antibody (Monkey) Kit: Required materials

• Vials
• Precision single and multichannel pipettes with disposable tips
• Adhesive sealing film or plates

Additional information

Data Sheet

 

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team