NoW-Dtech™ – Control Kit
Green – 545/520 nm

Description

The NoW-Dtech™ Control Kit 545/520 (Green) nm is designed to assess the compatibility of your TR-FRET reader with Poly-Dtech’s lanthanide-based technology.

It contains Bright-Dtech™ donor nanoparticles (Green) and an Acceptor that generates a TR-FRET signal upon binding, enabling you to optimize your reader parameters for maximum sensitivity and optimal signal-to-noise ratio.

 

Control kit: Protocol

 

Control kit: Validation

Control Kit: Required materials

• Vials
• Precision single and multichannel pipettes with disposable tips
• Adhesive sealing film or plates

Choosing the right plate reader allows to get the best possible results. NoW-Dtech™ products are compatible with TR-FRET plate readers.

For all technical support, commercial enquiries or further information, contact the Poly-Dtech team

 

Additional documents

• Data Sheet
• Security Sheet

 

Publications and references

Ultra-bright lanthanide nanoparticles
Terbium-doped LaF3 nanoparticles, surface-functionalized by photon-harvesting antenna ligands. Surface capping with antenna ligands leads to ultrabright nanoparticles, with the typical green luminescence signature of Tb atoms and very long excited-state lifetimes
Joan Goetz, Aline Nonat, Abdoulaye Diallo, Mohamadou Sy, Ildan Sera, Alexandre Lecointre, Christophe Lefevre, Chi Fai Chan, Ka-Leung Wong, Loïc J Charbonnière
Chempluschem. 2016 Jun;81(6):497.
doi: 10.1002/cplu.201600117

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007