Bright-Dtech™ 545 – Tb (Green)
– For LFIA –
Coupled to Anti-Human IgG

Description

Optimized for Lateral Flow Assays (LFIA) with Bright-Dtech™

With Bright-Dtech™ – For LFIA, we offer an innovative solution to significantly enhance detection sensitivity in Lateral Flow Assays (LFIA). Our fluorescent nano-molecules are specifically engineered for LFIA applications, ensuring high signal intensity, rapid detection, and superior contrast for accurate and reliable results (Watch our video on Youtube).

In LFIA, secondary antibodies play a crucial role in detecting and visualizing a primary antibody bound to its target analyte. For optimal specificity, a secondary anti-human antibody coupled to Bright-Dtech is used when detecting primary antibodies derived from human sources.

Thanks to the unique lanthanide fingerprint emission spectrum, Bright-Dtech™ 545 – Tb (Green) could be combined with other Bright-Dtech™ nanoparticles to enable multiplex detection of multiple biomarkers or proteins within the same LFIA strip, enhancing diagnostic capabilities without cross-reactivity.

 

How to Use Bright-Dtech ?

Recommended dilutions vary based on the type of assay, detection system, and sample type. Below are general guidelines:

Lateral Flow 

• Dilution range: 1:2 to 1:100
• Adjust according to signal intensity, background, and detection system sensitivity

 

We strongly recommend performing pilot titration experiments to determine the optimal working dilution under your specific conditions.

Need help or a custom antibody conjugate? Our team is here to help. Contact us at contact@poly-dtech.com

 

 

Requested Equipment

Additional Documents

• Data Sheet
• Security Sheet

 

Publications and references

Breaking the picomolar barrier in lateral flow assays using Bright-Dtech™ 614 – Europium nanoparticles for enhanced sensitivity,
Enhanced sensitivity in lateral flow assays using Poly-Dtech Europium nanoparticles specifically developed for LFIA applications
Juliette Lajoux, Yulieth D. Banguera-Ordoñez, Amadeo Sena-Torralba, Loïc J. Charbonnière, Mohamadou Sy, Joan Goetz, Ángel Maquieira, Sergi Morais
Microchemical Journal, 2025, Volume 209, 112864
doi: https://doi.org/10.1016/j.microc.2025.112864

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069