TR-FRET Assay Development
We develop custom TR-FRET assays using our advanced Bright-Dtech™ technology. Each assay undergoes a rigorous process to ensure exceptional quality and reproducibility.
Design your personalized TR-FRET assay or convert your ELISA assay into a streamlined mix-and-read test. TR-FRET eliminates washing steps (homogeneous test), significantly improving both the speed and sensitivity of detection.
EXPERTISE
Team of experts with strong skills in TR-FRET
EFFICIENCY
Time-saver and cost-effective to accelerate R&D process
FLEXIBILITY
Customization and adaptation to the most specific needs
Our range of services to suit all your specific needs:
- Development, optimization, validation and manufacturing of ready-to-use TR-FRET kits.
- Conversion of ELISA test into a TR-FRET test.
- Development of your test from your biological product.
- Routine testing of samples.
- Assay transfer and on-site training.
Design your test in 3 steps
Preliminary research
The first step in the TR-FRET assay development process is to define the project specifications and determine the best strategy to apply. One or more recommendations may be suggested based on:
- Type of test: Homogeneous / Heterogeneous TR-FRET
- Quantified/Detected Target: antibody, protein, biomolecules, oligonucleotide, peptide, small molecule, etc.
- The expected amount of antigen present
- Matrix: serum, plasma, culture supernatant, etc.
- Choice of primary material: samples, antigen, antibodies, etc.
Assay Optimization
Precise and optimal performance conditions are analyzed at each step of the test protocol. At this stage, we assess and optimize the key parameters and conditions, including the best acceptor, saturation, antigen capture, detection, incubation time and temperature, among others.
Assay Validation
Validating the method ensures that results are reproducible, reliable, robust, and accurate. The validation of the analytical method will be subjected to the following experimental checks:
- Specificity (the method must differentiate the targeted analyte from all other matrix components)
- Linearity (range, LLOQ)
- Sensitivity (LOD)
- Accuracy (spiking recovery test)
- Precision (repeatability = intra assay, inter assay; reproducibility = inter laboratory assay, or on different days or different plates)
- Robustness (defined as the ability to remain unaffected by small variations in method parameters, this encompasses factors such as incubation temperature, temperature of used components or variations in incubation time due to time delays during pipetting).
Your assay is now ready for your customers
Download Brochure on TR-FRET Assay Development
Contact us about your project
Since 2023, Poly-Dtech has been approved for the CIR (Research Tax Credit), enabling a 30% tax reduction for companies utilizing our R&D services. Find here the approval