Bright-Dtech^TM is based on lanthanides nanoparticles.

Lanthanides are chemical elements having the atomic number from Z from 57 (lanthanum) to 71 (lutetium). Their general electronic configuration is [Xe] 4fn5d16s2 where n is included between 0 to 14 and represents the number of electrons and [Xe] corresponds to the electronic configuration of the noble gas xenon. In aqueous solvents, lanthanide ions are under the state Ln3+ where they are the most stable in most cases. Each lanthanides elements have different optical and magnetic properties. Today, lanthanides are mainly used as catalysts or magnets. They are present in optical lasers, fibers, television, batteries for phones, computes or cars. In the last years, their specific properties have attracted life sciences and medical areas, especially for the detection of biomarkers.

The power of the nanoparticles resides in its nanometric structure. Nanoparticles are small objects between 1 to 100 nm and find applications in various sectors including healthcare, environment preservation, or cosmetic industry. Nanoparticles present offer many advantages such as their small size, large surface area for functionalization and good stability. They have many advantages, like small size, a large surface area for functionalization, and an excellent stability.

This technology offers many advantages such as high brightness, long emission lifetime, large Stokes shift and photostability compared to other fluorophores whose performances are moderate.

Based on lanthanides technology, Bright-Dtech^TM nanoparticles are 100-1000x brighter than Quantum dots, which allow a more accurate detection and reduce detection limits.

The high efficiency of the nanoparticles and their ease of use will enable them to replace current fluorescent molecules developed in the 2000s, which offer only moderate performance.

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With a large energy gap between the lowest excited state and the highest gournd level each lanthanide has a specific spectroscopic signature. The spectral range of lanthanides is large and they emit a strong luminescence:

-In the visible region (Sm³⁺, Eu³⁺, Tb³⁺, Dy³⁺)

-In the near infrared region (Nd³⁺, Er³⁺, Ho³⁺, Tm³⁺, Yb³⁺)

Bright-Dtech^TM ‘s brightness is based on 3 factors:

−Molar obsoprtion coefficient: Capacity of a molecule to absorb at a specific wavelength

−Quantum yield: Number of emitted photons per number of absorbed photons

−Brightness: With the luminescent quantum yield and the molar absorption coefficient, the brightness can be calculated by the following equation:

When a luminescent compound is excited, the excited state is populated and the return to the ground state results in the emission of light. This de-excitation intensity decreases exponentially with time, following the law:

• I₀ : Intensity after excitation
• I_em: Measured intensity at time t
• t : Time
• T : Excited-state lifetime

Lifetime is the time a fluorophore stays in its excited state. Conventional fluorescent nanomolecules have very short luminescent lifetimes. Lanthanide nanoparticles have an exceptionnally long lifetime, in the range of millisecond. Long lifetime presents many advantages: low background noise, high intensity of emission, high sensitivity, accurate measurement.

Yes, the scientific publications and references are the following:

−Ultra-bright lanthanide nanoparticles
Terbium-doped LaF3 nanoparticles, surface-functionalized by photon-harvesting antenna ligands. Surface capping with antenna ligands leads to ultrabright nanoparticles, with the typical green luminescence signature of Tb atoms and very long excited-state lifetimes
Joan Goetz, Aline Nonat, Abdoulaye Diallo, Mohamadou Sy, Ildan Sera, Alexandre Lecointre, Christophe Lefevre, Chi Fai Chan, Ka-Leung Wong, Loïc J Charbonnière
Chempluschem. 2016 Jun;81(6):497.
doi: 10.1002/cplu.201600117

−Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

−Live cell imaging without autofluorescence using terbium nanoparticles
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069

Link-Dtech^TM allows the conjugation of antibodies, proteins or any other biological entities.

Link-Dtech^TM is designed to couple antibodies and other biologicals. If you need a specific coupling (COOH, NH2, N3…), contact us.

Link-Dtech^TM allows to conjugate biological entities with Bright-Dtech^TM without complex coupling chemistry or having access to specialized chemistry capabilities or specific equipments.

Youtube video: https://youtu.be/21HC5tL9Mv4

Link-Dtech^TM kits include the nanoparticles, the dilution buffer and the coupling buffer.

With Link-Dtech^TM, it is possible to couple between 10 and 200 antibodies per nanoparticles.

The yield is more than 90%. And the number of antibodies around the nanoparticle can be checked by TR-FRET.

It is strongly recommended not to use PBS Buffer with Link-Dtech^TM .

There are many validation steps:

• To check that there is no loss of nanoparticles, it is possible to check with the UV lamp
• If Link-Dtech^TM is coupled with an antibody, it is possible to verify with lateral flow strips or FLISA. If the conjugation was made with other biological samples, contact us.

Link-Dtech^TM and all the components must be stored at 4°C.

The shelf life of Link-Dtech^TM is 1 year.

Time-resolved fluorescence energy transfer (TR-FRET) represents the practical fusion of Time-Resolved Fluorescence (TRF) with Förster Resonance Energy Transfer (FRET) offering a powerful tool researchers in drug discovery. TR-FRET combines the low-background nature of TRF with the homogeneous assay format of FRET. The combined assay offers increased flexibility, reliability and sensitivity along with higher throughput and a reduced occurence of false-positive and false-negative results. FRET involves two fluorophores, a donor and an acceptor. When the donor is excited by an energy source (such as flash lamp or laser), it transfers energy to the acceptor if they are within a specific proximity to each other. Subsequently, the acceptor emits light at its characteristic wavelength.

Bright-Dtech™ technology brings a more sensitive and stable approach due to an improved limit of detection thanks to the brightness, a high photostability and a long emission lifetime.

For an optimal use of NoW-Dtech^TM, please follow the whole data sheet of the assay kit.

For all technical support or further information, please contact us.

NoW-Dtech^TM includes Standard Stock, Bright-Dtech™ Donor antibody, Acceptor antibody, TRF Buffer and a 96-well black microplate.

• Increased Sensitivity: Thanks to the energy resonance between two fluorophores, the FRET method can detect molecular interactions, enabling more accurate detection of biomarkers, even in samples with low concentrations.
• Dynamic Response: Unlike ELISA, the FRET method provides a real-time dynamic response. This capability to monitor interactions in real time yields valuable data on molecular interactions, leading to a better understand of binding mechanisms and biological processes.
• Lower Cost: TR-FRET requires fewer reagents, consumables and a smaller sample volume, thereby reducing collection and storage costs
• Time-Saving: The FRET technique is faster than the ELISA one, simplifying the process by eliminating certain complex antibody-related steps.
• No Washing Steps: Additionally, it does not require washing steps. This speed allows for the execution of more experiments within a given timeframe.

Fluorescence-Linked Immunosorbent Assay (FLISA) is an analytical method which can be used for the detection and quantification of specific proteins, biomolecules, peptides, cytokines, hormones, antibodies and more present in serum, plasma and cell culture supernatant. As TR-FRET, this method is based on Time-Resolved Fluorescence (TRF) and is a variant of the ELISA technique, replacing the enzymatic reaction with a fluorescence signal.

In Bright-Dtech™ FLISA method, detection/secondary antibodies are conjugated to fluorescent nanoparticles, the fluorescent intensity is measured by exposing the plate to light at a specific wavelength using time-resolved fluorescence reader, thereby enhancing the sensitivity of the analysis.

There are two common types of FLISA, namely Direct/Indirect FLISA and Sandwich FLISA. In Indirect FLISA, a Bright-Dtech-conjugated secondary antibody against the host species of the primary antibody is used for detection. In Direct FLISA, antibodies are directly conjugated to Bright-Dtech nanoparticles. In Sandwich FLISA, the detection antibody is linked to the Bright-Dtech™ fluorescent nanoparticles, enabling accurate and specific analyte quantification with minimal background noise. This final step can be performed using either direct or indirect methods (requiring a third antibody) for sandwich detection.

For an optimal use of FLISA-Dtech^TM, please follow the whole data sheet of the assay kit.

For all technical support or further information, please contact us.

FLISA-Dtech^TM includes Standard Stock, BDT Detection Antibody (200X), Wash Buffer (10X), Dilution Buffer, Assay Buffer and a 96-well black microplate (12 strips of 8 wells).

• Fast: Fluorescent reactions can be detected within minutes, in contrast to the longer time required by ELISA. This technique eliminates the need for enzymatic revelation.
• Enhanced Sensitivity: Fluorescence can detect lower concentrations compared to the ELISA method, thanks to more efficient fluorescent makers such as lanthanide nanoparticles
• Expanded Dynamic Range: The FLISA method can measure a broader range of concentrations without the need for frequent dilutions.
• Reduced Sample and Reagent Usage: FLISA often requires smaller sample volumes compared to ELISA. Additionally, it consumes fewer reagents, leading to cost and chemical consumption reduction.
• No Enzymatic Revelation Required: The FLISA method eliminates the need for enzymatic revelation, saving time and preventing cross-reactions between samples and enzymes.

We provide three types of services:

• Development, Validation and Manufacturing of a ready-to-use kits
• Enhancement of your test or conversion of your ELISA test into a TR-FRET or TR-FLISA assay
• Development of Nanoparticles in various sizes

1.Preliminary Research: The initial step in the development involves defining the project specifications and the most suitable strategy. This includes determining the test type, the target to be quantified, the expected amount of antigen, the matrix, and the choice of primary materials.

2.Optimization: Subsequently, we focus on studying precise and optimal performance conditions at each step of the test protocol. During this phase, we assess and optimize various parameters and conditions such as the selection of the best acceptor, saturation, antigen capture, detection, incubation time and temperature, among others.

3.Validation: The validation phase is essential to ensure that the method provides consistent, reliable, robust, and accurate results. The validation of the analytical method involves conducting the following experimental checks:

• Specificity
• Linearity (range, LLOQ)
• Sensitivity (LOD)
• Accuracy (spking recovery test)
• Precision (repeatability and reproducibility)
• Robustness

Yes, since 2023, Poly-Dtech is CIR (Crédit Impôt Recherche) approved, allowing a 30% tax reduction for companies utilizing our R&D services (antibody conjugation and custom assay development) for the next three years.

Poly-Dtech is a French company, based in Strasbourg, specializing in the field of life sciences and in vitro diagnostics (IVD). We specialize in the development and production of high-performance fluorescent nanoparticles and immunoassays, each with unique characteristics designed to enhance the detection of biomarkers for scientific research. Our products offer superior detection sensitivity through user-friendly protocols applicable in bioanalytical methods (immunoassays) and various molecular and cellular biology techniques.

Poly-Dtech’s technology is the culmination of many years of work and collaboration among experts in nanoparticle synthesis, spectroscopy, biology and analysis techniques, both in France and around the world.

Through our technology, our aim is to advance current screening, drug discovery and early disease diagnosis, ultimately improving the quality of life for patients and expanding treatment options.

Poly-Dtech’s team are experts in chemistry and biology between France and Hong-Kong. This multi-disciplinary approach, coupled with a multicultural background, not only provides the required skills and experiences, but also encompasses a diverse set of values, beliefs and perspectives. This diversity fosters a unique environment for innovation.

Poly-Dtech’s website has an e-commerce shop. Thus, you can place an order of Bright-Dtech^TM, Link-Dtech^TM, NoW-Dtech or FLISA-Dtech here.

Our kits can be sent within 5 working days all around the world.