Lateral Flow Assay Development
Poly-Dtech develops quantitative Lateral Flow tests that provide performance equivalent to an ELISA test, undergoing a comprehensive and rigorous process to ensure exceptional quality and reproducibility.
Develop your personalized rapid test through our advanced Bright-Dtech™ technology for a sensitivity 100 times greater than current rapid tests.
EXPERTISE
Team of experts with strong skills in Lateral Flow
EFFICIENCY
Time-saver and cost-effective to accelerate R&D process
FLEXIBILITY
Customization and adaptation to the most specific needs
Lateral flow assays offer versatile applications not only in human health diagnostics but also in:
- Veterinary field
- Food safety
- Environmental safety
- Pharmaceutical industries
- Industrial control quality
- Defense and security
Preliminary Research
The first step of the assay development process is to define the specifications of the project and the best strategy to be applied. One or more recommendations could be proposed in function of:
- Type of test: serologic or antigenic (competitive or sandwich formats).
- Targeted analyte: antibody, protein, biomolecules, oligonucleotide, peptide, small molecule, etc.
- Types of readouts: Quantitative, Semi-quantitative, qualitative.
- Matrix: serum, plasma, urine, saliva, water, etc.
- Evaluation and selection of the best components: membrane, strip, buffer, antigens, bioreceptor (proteins, antibodies, or DNA).
Assay Optimization
Precise and optimal performance conditions are studied for each step within the lateral flow assay protocol. At this point, we evaluate and optimize the best strip materials (nitrocellulose membrane, conjugate, sample, absorbent pads) and parameters (buffers, blocking reagents, capture bioreceptors and detection bioreceptors concentration, time of migration, stripping condition)
Assay Validation
Validation of the method to ensure reproducible, reliable, robust, and accurate results. Validation of the analytical method will be subjected to the following experimental checks:
- Specificity (the method must differentiate the targeted analyte from all other matrix components)
- Linearity (range, LLOQ)
- Sensitivity (LOD)
- Accuracy (spiking recovery test)
- Precision (repeatability = intra assay, inter assay; reproducibility = inter laboratory assay, or on different days or different plates) Robustness (defined as the ability to remain unaffected by small variations in method parameters. These parameters could be incubation temperature, temperature of used components or varied incubation time due to time delay during pipetting).
Experience unparalleled performance with our LF reader, leveraging cutting-edge Bright-Dtech technology to enhance detection sensitivity by 100 times!
Download Brochure on Lateral Flow Assay Development
Contact us about your project
Since 2023, Poly-Dtech is CIR (Research Tax Credit) approved, allowing a tax reduction of 30% for companies that call on our R&D services for the next three years. Find here the approval