Research & Discovery

TR-FRET is an advanced, no-wash fluorescence resonance energy transfer technology designed to provide a faster and highly sensitive alternative to traditional ELISA. Poly-Dtech’s TR-FRET technology enables precise biomarker quantification, protein-protein interaction studies, and high-throughput screening with exceptional accuracy. By leveraging energy transfer between donor and acceptor molecules in close proximity, TR-FRET generates a stable, time-resolved signal with minimal background interference.

TR-FRET assay kits – Pre-configured kits designed for the quantification of biomarkers, cytokines, and phosphorylated proteins with high sensitivity and reproducibility.

Key advantages of Poly-Dtech’s TR-FRET technology:

• Faster results with no-wash protocol
• High sensitivity for detecting low-abundance analytes
• Enhanced signal stability and reduced background noise
• Compatibility with diverse sample types, including cell lysates, serum, plasma, and tissue extracts

Watch an example of protocol on YouTube for a step-by-step guide to using our technology ➔ Click here to view the video

Explore Poly-Dtech’s TR-FRET technology to accelerate your research with superior performance and efficiency.

Poly-Dtech’s Multiplex TR-FRET technology is a groundbreaking fluorescence-based assay platform, uniquely available worldwide, designed for the simultaneous detection of multiple targets in a single sample. Combining the high sensitivity and stability of TR-FRET with multiplexing capabilities, this technology enables the quantification of 2 to 12 analytes with exceptional precision and minimal background interference.

Multiplex TR-FRET retains the no-wash protocol and time-resolved fluorescence detection of traditional TR-FRET assays, while significantly increasing throughput and data richness. This makes it an ideal solution for high-content screening, biomarker discovery, and pathway analysis in both research and diagnostic applications.

Key advantages of Poly-Dtech’s multiplex TR-FRET:

• World’s only solution for TR-FRET multiplexing from 2 to 12 targets
• No-wash protocol for faster and more efficient workflows
• High sensitivity for detecting low-abundance biomarkers
• Time-resolved fluorescence for reduced background noise and enhanced signal stability
• Broad sample compatibility, including cell lysates, serum, plasma, and tissue extracts
• Multiplexing capabilities for comprehensive pathway analysis and biomarker profiling

Unlock the power of high-throughput, multi-analyte detection with Poly-Dtech’s exclusive multiplex TR-FRET technology to enhance your research and accelerate discoveries.

FLISA-Dtech is a highly sensitive, wash-based fluorescence immunoassay technology that enhances traditional ELISA methods by incorporating stable, time-resolved fluorescence detection. Developed by Poly-Dtech, this platform offers superior assay performance with an extended dynamic range, allowing for precise quantification of biomarkers and analytes across diverse applications.

FLISA-Dtech retains the workflow simplicity of ELISA while delivering enhanced signal stability and sensitivity. It supports multiplexing, enabling the simultaneous detection of multiple targets in a single sample. This versatility makes it an ideal choice for high-throughput screening, biomarker discovery, and diagnostic applications.

Key advantages of FLISA-Dtech:

• Stable, time-resolved fluorescence for improved signal accuracy
• Enhanced assay dynamic range for detecting low and high concentrations
• Wash-based format ensuring high specificity and reduced background
• Multiplexing capabilities for simultaneous multi-analyte detection
• Broad sample compatibility, including buffers, cell supernatants, lysates, serum, plasma, and tissue extracts

Experience the precision and reliability of FLISA-Dtech to elevate your immunoassay performance with cutting-edge fluorescence technology.

At Poly-Dtech, we offer a comprehensive suite of services tailored to support your research and diagnostic needs. From custom assay development to licensing and training, our solutions are designed to deliver precision, flexibility, and superior performance.

Assay development

• FLISA-Dtech assay development:
  Customizable, high-sensitivity, wash-based fluorescence immunoassays tailored to your specific biomarker detection needs. Benefit from an extended dynamic range and robust performance across various sample types ➔ Read more
• TR-FRET assay development:
  Design and optimize time-resolved FRET assays with expert support, ensuring precise, reproducible results for protein-protein interaction studies, biomarker quantification, and more ➔ Learn more
• Multiplex TR-FRET assay development:
  Develop advanced multiplex assays capable of detecting 2 to 12 analytes in a single sample. Maximize throughput and data output with our unique TR-FRET multiplexing technology ➔ See how it works

OEM Solutions:
Partner with Poly-Dtech to incorporate our advanced assay technologies into your product offerings. We provide customized OEM solutions tailored to your specifications, ensuring seamless integration and superior performance

Licensing:
Access Poly-Dtech’s proprietary technologies for integration into your own platforms and products. Flexible licensing agreements are available to meet your business needs

Training:
Enhance your team’s expertise with hands-on training sessions and workshops led by our technical experts. Learn how to implement and optimize our FLISA-Dtech, TR-FRET, and Multiplex TR-FRET technologies

Our Bright-Dtech kits feature ultra-fluorescent nanoparticles specifically designed for enhanced signal detection and superior sensitivity in a wide range of assays. Leveraging advanced lanthanide-based technology, these nanoparticles offer exceptional brightness, photostability, and low background interference, making them ideal for both qualitative and quantitative applications.

The nanoparticles are pre-conjugated with high-affinity IgG antibodies to ensure optimal performance and ease of use.

Available conjugations include:

• Anti-mouse IgG

• Anti-rabbit IgG

• Anti-goat IgG

• Anti-human IgG

• Streptavidin

• Biotin

These pre-coupled nanoparticles are pre-optimized for immediate use, eliminating the need for complex conjugation steps. This ensures consistent, reliable, and reproducible results, even in high-throughput workflows. Whether you’re working in biomarker detection, immunoassays, flow cytometry, or imaging applications, Bright-Dtech kits deliver robust performance tailored to meet the most demanding research requirements.

For Lateral Flow and Rapid Test applications, we offer a specific nanoparticle formulation optimized for enhanced performance in these formats. This dedicated nanoparticle type is readily available to meet the unique sensitivity and stability requirements of rapid diagnostic tests.

Key benefits include:

• Ultra-high fluorescence intensity for increased sensitivity

• Stability under harsh conditions, ensuring long-term reliability

• Ready-to-use, pre-conjugated nanoparticles for simplified workflows

• Broad compatibility with ELISA, FRET, and multiplex assays

With Bright-Dtech kits, you can confidently accelerate your research, achieve precise data, and enhance the overall efficiency of your diagnostic or discovery workflows.

Link-Dtech Kits provide advanced nanoparticle conjugation solutions, allowing you to easily couple biomolecules—such as antibodies, proteins, peptides, nucleic acids, and other ligands—to our nanoparticles for custom assay development. These kits are designed to ensure high coupling efficiency (up to 90%) and optimal stability, enabling reproducible results across various experimental conditions.

Our conjugation technology supports both covalent and non-covalent binding, offering flexibility depending on your assay requirements. Whether you are working on immunoassays, biosensors, flow cytometry, or diagnostic platforms, Link-Dtech Kits simplify the labeling process while maintaining the biological activity of your molecules.

Key features include:

• Versatile coupling protocols compatible with a wide range of biomolecules

• High-efficiency conjugation for strong, stable nanoparticle-biomolecule interactions

• Optimized for consistency, reducing batch-to-batch variability

• Suitable for diverse applications, from R&D to diagnostic assay development

With Link-Dtech kits, you can streamline your assay development, save valuable time, and achieve reliable, high-performance results tailored to your specific research goals. Discover our protocol (see below) or our Youtube video ➔ Watch now

We offer a comprehensive selection of high-affinity antibodies. Our extensive antibody library includes a wide range of monoclonal and polyclonal antibodies targeting key biomarkers across various research applications. Each antibody is rigorously validated to ensure high specificity, sensitivity, and reproducibility, making them ideal for immunoassays, flow cytometry, western blot, and more.

In addition to our advanced nanoparticle-based solutions, we also provide classic ELISA kits using HRP (Horseradish Peroxidase) detection technology. These cost-effective yet high-quality kits are designed for reliable and easy quantification of biomolecules, offering a trusted alternative for researchers looking for affordability without compromising performance.

• List of our top 50 of antibodies
• List of our top 100 of ELISA kits

Whether you need cutting-edge fluorescent nanoparticle-based assays or traditional ELISA kits, our catalog ensures that you find the right solution to match your research or diagnostic needs.

Tailor your assays with our custom nanoparticle conjugation and labeling services, designed to meet the unique needs of your research and diagnostic projects. Our team of experts works closely with you to develop high-performance reagents optimized for sensitivity, stability, and specificity.

We offer flexible conjugation strategies, including both covalent and non-covalent binding techniques, to ensure strong and stable attachment of biomolecules such as antibodies, proteins, peptides, nucleic acids, and other ligands to our nanoparticles. Whether you require small-scale R&D batches or large-scale production, our services are adaptable to your project’s scale and complexity.

What we offer:

• Personalized consultation to define the best conjugation approach for your application

• Optimization of coupling conditions to maximize binding efficiency and preserve biomolecule functionality

• Wide compatibility with different assay formats, including ELISA, lateral flow assays, FRET, and flow cytometry

• Quality control with rigorous validation to ensure batch-to-batch consistency and reproducibility

Partnering with us means benefiting from state-of-the-art nanoparticle technology and expert scientific support, ensuring your assays deliver reliable, accurate, and reproducible results every time.

Licensing:
Gain access to Poly-Dtech’s proprietary technologies through flexible licensing agreements designed to support your innovation and commercial goals.

Training:
Benefit from specialized training programs that cover the implementation, optimization, and troubleshooting of our assay technologies.

OEM Solutions:
Integrate Poly-Dtech’s advanced technologies into your products with our customized OEM solutions, designed to ensure seamless performance and reliability.