Link-Dtech™ 880 – Nd (Infrared)

Description

With Link-Dtech™, we provide an innovative solution that delivers enhanced detection sensitivity through easy-to-use protocols, compatible with a wide range of assays and molecular or cellular biology techniques. These ultra-efficient fluorescent nanomolecules possess unique characteristics that make them ideal for even the most demanding applications.

Whether you’re developing a custom detection assay or optimizing an existing protocol, Link-Dtech™ offers unmatched sensitivity and flexibility, combined with a straightforward and reliable conjugation workflow.

Poly-Dtech ensures a simple and efficient coupling process. Each kit is designed for the specific conjugation of an analyte, antibody, or other biomolecules. In addition, the nanoparticle surface can be customized to suit your experimental requirements.

 

➡️ Coupling capacity:

With 200 µL of Link-Dtech™, you can typically couple between 15 and 50 µg of antibody, depending on your desired nanoparticle-to-antibody ratio. The optimal amount depends on how densely you wish to coat the nanoparticle surface, and on the size (molecular weight) of the biomolecule.

For example:

• For a standard IgG antibody (~150 kDa), this amount allows coupling of approximately 50 to 200 antibody molecules per nanoparticle, depending on how densely you wish to decorate the surface.
• For a smaller protein, such as streptavidin (~60 kDa), a similar mass (15–50 µg) would correspond to a larger number of protein molecules per nanoparticle, as the molecular weight is lower.
• For larger biomolecules, such as full-length enzymes (~250–300 kDa), fewer molecules will be coupled per nanoparticle for the same total mass.

 

These values can be adjusted according to your application (signal amplification, steric hindrance, etc.), and custom optimization is available.

If you need assistance with a specific coupling strategy, don’t hesitate to contact us: contact@poly-dtech.com

For more information, please watch the tutorial video “Link-Dtech – How to use?” on YouTube:🔗 https://youtu.be/21HC5tL9Mv4

 

 

Requested Equipment

Additional Documents

• Data Sheet
• Security Sheet

 

Publications and references

Ultra-bright lanthanide nanoparticles
Terbium-doped LaF3 nanoparticles, surface-functionalized by photon-harvesting antenna ligands. Surface capping with antenna ligands leads to ultrabright nanoparticles, with the typical green luminescence signature of Tb atoms and very long excited-state lifetimes
Joan Goetz, Aline Nonat, Abdoulaye Diallo, Mohamadou Sy, Ildan Sera, Alexandre Lecointre, Christophe Lefevre, Chi Fai Chan, Ka-Leung Wong, Loïc J Charbonnière
Chempluschem. 2016 Jun;81(6):497.
doi: 10.1002/cplu.201600117

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069