Bright-Dtech™ 880 – Nd (Infrared)
Coupled to Anti-Human IgG (H + L)

Description

With Bright-Dtech™, we offer you an innovative solution to have a higher detection sensitivity with easy-to-use protocols, applicable in various molecular and cellular biology techniques. They are ultra-efficient fluorescent nano-molecules with unique characteristics.

Secondary antibodies aim to detect and visualize a primary antibody that is bound to its protein of interest. Human antibody is often used in applications such as ELISA, Western Blot, IHC, or cell imaging.

If you are using a primary antibody produced in humans, you will choose as the secondary antibody an anti-human antibody produced in a species other than human.

Due to lanthanide’s fingerprinting emission spectrum, Bright-Dtech™ – Nd (Infrared) can be incorporated with other Bright-Dtech™ nanoparticles to achieve multiple detection of biomarkers or proteins in the same biological sample.

 

How to Use Bright-Dtech ?

Recommended dilutions vary based on the type of assay, detection system, and sample type. Below are general guidelines:

 Immunoassays (e.g., TR-FRET, FLISA, dot-blot)

• Dilution range: 1:100 to 1:1000
• Adjust according to signal intensity, background, and detection system sensitivity

 

Cell-based assays (e.g., flow cytometry, immunofluorescence, imaging)

• Dilution range: 1:5 to 1:20 (or higher)
• Optimization depends on:
  – Instrument sensitivity (e.g., time-resolved plate readers, confocal microscopes, flow cytometers)
  – Sample type (fixed vs live cells, tissue slices)
  – Blocking and washing conditions
  – Signal-to-noise requirements

 

We strongly recommend performing pilot titration experiments to determine the optimal working dilution under your specific conditions.

Need help or a custom antibody conjugate? Our team is here to help. Contact us at contact@poly-dtech.com

 

 

Requested Equipment

Additional Documents

• Data Sheet
• Security Sheet

 

Publications and references

Ultra-bright lanthanide nanoparticles
Terbium-doped LaF3 nanoparticles, surface-functionalized by photon-harvesting antenna ligands. Surface capping with antenna ligands leads to ultrabright nanoparticles, with the typical green luminescence signature of Tb atoms and very long excited-state lifetimes
Joan Goetz, Aline Nonat, Abdoulaye Diallo, Mohamadou Sy, Ildan Sera, Alexandre Lecointre, Christophe Lefevre, Chi Fai Chan, Ka-Leung Wong, Loïc J Charbonnière
Chempluschem. 2016 Jun;81(6):497.
doi: 10.1002/cplu.201600117

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069