Bright-Dtech™ 880 – Nd (Infrared)
Coupled to Biotin

Description

With Bright-Dtech™, we offer you an innovative solution to have a higher detection sensitivity with easy-to-use protocols, applicable in various molecular and cellular biology techniques. They are ultra-efficient fluorescent nano-molecules with unique characteristics.

Biotin is a co-enzyme which has a strong affinity with Streptavidin. Our experts have therefore been able to take advantage of the link to offer you a product that meets your expectations which can be used in many detection protocols such as protein assays on microplates, Western Blot, flow cytometry, imaging and microscopy.

Due to lanthanide’s fingerprinting emission spectrum, Bright-Dtech™ – Nd (Infrared) can be incorporated with other Bright-Dtech™ nanoparticles to achieve multiple detection of biomarkers or proteins in the same biological sample.

 

How to Use Bright-Dtech ?

Recommended dilutions vary based on the type of assay, detection system, and sample type. Below are general guidelines:

 Immunoassays (e.g., TR-FRET, FLISA, dot-blot)

• Dilution range: 1:100 to 1:1000
• Adjust according to signal intensity, background, and detection system sensitivity

 

Cell-based assays (e.g., flow cytometry, immunofluorescence, imaging)

• Dilution range: 1:5 to 1:20 (or higher)
• Optimization depends on:
  – Instrument sensitivity (e.g., time-resolved plate readers, confocal microscopes, flow cytometers)
  – Sample type (fixed vs live cells, tissue slices)
  – Blocking and washing conditions
  – Signal-to-noise requirements

 

We strongly recommend performing pilot titration experiments to determine the optimal working dilution under your specific conditions.

Need help or a custom antibody conjugate? Our team is here to help. Contact us at contact@poly-dtech.com

 

 

Requested Equipment

Additional Documents

• Data Sheet
• Security Sheet

 

Publications and references

Ultra-bright lanthanide nanoparticles
Terbium-doped LaF3 nanoparticles, surface-functionalized by photon-harvesting antenna ligands. Surface capping with antenna ligands leads to ultrabright nanoparticles, with the typical green luminescence signature of Tb atoms and very long excited-state lifetimes
Joan Goetz, Aline Nonat, Abdoulaye Diallo, Mohamadou Sy, Ildan Sera, Alexandre Lecointre, Christophe Lefevre, Chi Fai Chan, Ka-Leung Wong, Loïc J Charbonnière
Chempluschem. 2016 Jun;81(6):497.
doi: 10.1002/cplu.201600117

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069