Bright-Dtech™ 614 – Eu (Red)
– For LFIA –
Coupled to Anti-Mouse IgG

Description

Optimized for Lateral Flow Assays (LFIA) with Bright-Dtech™

With Bright-Dtech™ – For LFIA, we offer an innovative solution to significantly enhance detection sensitivity in Lateral Flow Assays (LFIA). Our fluorescent nano-molecules are specifically engineered for LFIA applications, ensuring high signal intensity, rapid detection, and superior contrast for accurate and reliable results.

In LFIA, secondary antibodies play a crucial role in detecting and visualizing a primary antibody bound to its target analyte. To ensure optimal specificity, Bright-Dtech™ 614 – Eu (Red) is coupled to an anti-mouse IgG antibody, making it the ideal choice for detecting mouse-derived primary antibodies.

Thanks to the unique lanthanide fingerprint emission spectrum, Bright-Dtech™ 614 – Eu (Red) could be combined with other Bright-Dtech™ nanoparticles to enable multiplex detection of multiple biomarkers or proteins within the same LFIA strip, enhancing diagnostic capabilities without cross-reactivity.

 

How to Use Bright-Dtech ?

Recommended dilutions vary based on the type of assay, detection system, and sample type. Below are general guidelines:

Lateral Flow 

• Dilution range: 1:2 to 1:100
• Adjust according to signal intensity, background, and detection system sensitivity

We strongly recommend performing pilot titration experiments to determine the optimal working dilution under your specific conditions.

Need help or a custom antibody conjugate? Our team is here to help. Contact us at contact@poly-dtech.com

 

 

Requested Equipment

Additional Documents

• Data Sheet
• Security Sheet

 

Publications and references

Breaking the picomolar barrier in lateral flow assays using Bright-Dtech™ 614 – Europium nanoparticles for enhanced sensitivity,
Enhanced sensitivity in lateral flow assays using Poly-Dtech Europium nanoparticles specifically developed for LFIA applications
Juliette Lajoux, Yulieth D. Banguera-Ordoñez, Amadeo Sena-Torralba, Loïc J. Charbonnière, Mohamadou Sy, Joan Goetz, Ángel Maquieira, Sergi Morais
Microchemical Journal, 2025, Volume 209, 112864
doi: https://doi.org/10.1016/j.microc.2025.112864

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069