NoW-Dtech™ – TR-FRET Assay Kits
NoW-Dtech™ TR-FRET Assay Kits are an advanced solution engineered for accurate and reliable detection of specific biomarkers or molecules in biological samples. TR-FRET combines TRF (Time-resolved Fluorescence) for minimized background signal with FRET (Förster Resonance Energy Transfer) to enable homogeneous assays.
Energy transfer occurs due to the proximity of two light-sensitive molecules (a donor and an acceptor) and is facilitated by the competitive wavelengths of the two chromophores involved. Based on Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology, our NoW-Dtech™ TR-FRET Assay Kits provide unparalleled sensitivity and specificity for your research applications.
Employing our Bright-Dtech™ as a donor, background noise will be reduced. Indeed, our lanthanides-based fluorescent nanoparticles are characterized by an extended lifetime, emitting fluorescence for several milliseconds. Thus, a delay can be introduced between the excitation/emission of the nanoparticle and the reading. Thanks to this delay, the background noise is reduced, and the reliability enhanced.
By combining FRET and our technology, our NoW-Dtech™ TR-FRET Assay Kits are an analytical method which can be used for the detection and the quantification of specific proteins, biomolecules, peptides, cytokines, hormones or antibodies, present in serum, plasma or cell culture supernatant. They present unique characteristics:
- Wide Dynamic Range: With its broad dynamic range, NoW-Dtech™ allow the quantification of both high and low concentrations of target molecules, ensuring accurate results across a wide range of sample concentrations
- High Performance: NoW-Dtech™ supress background noise and provide reliable data for your assays
- Ease of Use: NoW-Dtech are ready-to-use with clear step-by-step instructions, making it suitable for both experienced researchers and newcomers to TR-FRET Assay Kits
TR-FRET Assay Kits find extensive application in various research areas, including:
- Biomarker identification and validation
- Drug discovery and development
- Protein-protein interactions
- Signal transduction studies
Discover our range of ready-to-use TR-FRET Assay Kits:
Human IgG
Ref: FRET-hIgG
- LOD: 12 pg/mL
- Assay range: 0.05 – 1200 ng/mL
- Incubation time: 2h30
- Specificity: Human IgG
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Mouse IgG
Ref: FRET-mIgG
- LOD: 2 ng/mL
- Assay range: 1 – 1000 ng/mL
- Incubation time: 1h30
- Specificity: Mouse IgG
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Human IgM
Ref: FRET-hIgM
- LOD: 12 pg/mL
- Assay range: 0.05 – 1200 ng/mL
- Incubation time: 2h30
- Specificity: Human IgM
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Human CRP
Ref: FRET-hCRP
- LOD: 110 pg/mL
- Assay range: 600 – 100 000 pg/mL
- Incubation time: 1h30
- Specificity: Human CRP
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Anti-HER2 antibody (Monkey)
Ref: FRET-mkTrastu
- LOD: 75 pg/mL
- Assay range: 11.7 – 3000 ng/mL
- Incubation time: 1h30
- Specificity: Anti-HER2 antibodies (Monkey)
Each kit is provided in a 384-well plate format
And includes 1, 2, 5, or 10 plates — with larger quantities available upon request.
Control Kit 545/520 nm
Ref: FRET-CKgreen
The NoW-Dtech™ Control Kit 545/525 nm is designed to assess the compatibility of your TR-FRET reader (green parameters) with Poly-Dtech’s lanthanide-based technology.
Each kit is provided in a 384-well plate format
Control Kit 616/665 nm
Ref: FRET-CKred
The NoW-Dtech™ Control Kit 616/665 nm is designed to assess the compatibility of your TR-FRET reader (red parameters) with Poly-Dtech’s lanthanide-based technology.
Each kit is provided in a 384-well plate format
We are currently developing new tests in our R&D center, visit our website regularly to discover them.