Bright-Dtech™ Starter Pack

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SKU: BDTSP Category: Tags: ,

Description

The Starter Pack have been designed to try our technology Bright-Dtech™ at a low price. The concentration and the volume are adapted to make many experiments in order to observe the advantages of our nanoparticles and to improve your results.

To know everything about our technology, you can visit the page : https://poly-dtech.com/technology-and-applications/technology/

With Bright-Dtech™, we offer you an innovative solution to have a higher detection sensitivity with easy-to-use protocols, applicable in various molecular and cellular biology techniques. They are ultra-efficient fluorescent nano-molecules with unique characteristics.

Bright-Dtech™ is a patented technology based on lanthanide nanoparticles that provides exceptional performance in a wide range of fluorescent analysis applications. We increase the performance of current fluorescent markers by our nanoparticles in different applications (Immunoassays, cell imaging, flow cytometry, lateral flow, dot Blot).

Due to lanthanide’s fingerprinting emission spectrum, Bright-Dtech™ – Tb (Green) & Bright-Dtech™ – Eu (Red) can be incorporated with other Bright-Dtech™ nanoparticles to achieve multiple detection of biomarkers or proteins in the same biological sample.

Requested Equipment

Additional Documents

 

Publications and references

Ultra-bright lanthanide nanoparticles
Terbium-doped LaF3 nanoparticles, surface-functionalized by photon-harvesting antenna ligands. Surface capping with antenna ligands leads to ultrabright nanoparticles, with the typical green luminescence signature of Tb atoms and very long excited-state lifetimes
Joan Goetz, Aline Nonat, Abdoulaye Diallo, Mohamadou Sy, Ildan Sera, Alexandre Lecointre, Christophe Lefevre, Chi Fai Chan, Ka-Leung Wong, Loïc J Charbonnière
Chempluschem. 2016 Jun;81(6):497.
doi: 10.1002/cplu.201600117

Ultrabright Terbium Nanoparticles for FRET Biosensing and In Situ Imaging of Epidermal Growth Factor Receptors
Design of a ligand that can be simultaneously applied as an efficient light-harvesting antenna for Tb surface ions and a strong binder of biomolecules to LnNPs surfaces.
Cyrille Charpentier, Vjona Cifliku, Joan Goetz, Aline Nonat, Clémence Cheignon, Marcelina Cardoso Dos Santos, Laura Francés-Soriano, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt
Chemistry. 2020 Nov 17;26(64):14602-14611.
doi: 10.1002/chem.202002007

Live cell imaging without autofluorescence using terbium nanoparticles
Application of a new type of surface photosensitized terbium NPs (Tb-NPs) for autofluorescence-free intracellular imaging in live HeLa cells. Combination of exceptionally high brightness, high photostability, and long photoluminescence (PL) lifetimes for highly efficient suppression of short-lived autofluorescence, enabling timed PL imaging of intracellular vesicles over 72 h without toxicity and at extremely low concentrations of Tb-NP for up to 12h.
Joan Goetz, Marcelina Cardoso Dos Santos, Ka-Leung Wong, Loïc J Charbonnière, Niko Hildebrandt, Hortense Bertenlian
Bioconjugate Chem. 2018, 29, 4, 1327–1334.
doi: 10.1021/acs.bioconjchem.8b00069

Additional information

Weight 0.3 kg
Dimensions 20 × 6 × 1 cm
Volume

Concentration

Coupled to

, , , , , ,

Wavelength

340ex. – 545em., 340ex. – 614em.

Color

Green, Red

Nanoparticle

Europium (Eu), Terbium (Tb)

Product

Bright-Dtech